What is the minimum Ct value that you would consider indicates no gene expression?
asked 37 days ago
0
14
I am doing relative quantification to compare gene expression between a control with several mutant backgrounds using qPCR.
Looking online I find several different sources suggesting different values that indicate high, low, and no gene expression. Some say that Ct > 35 = no expression, others say > 40 = no expression.
Where do you draw the line with Ct values and why?
0 Answers
No Answers Found
The answer board is empty. Make it rain with your brilliant answer.
1
