How Does CRISPR-Cas9 Achieve Targeted Gene Editing?

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asked 37 days ago

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CRISPR-Cas9 has revolutionized genetic engineering by enabling precise modifications of DNA sequences.

I’m looking to understand the step-by-step molecular process, including the role of guide RNAs and the Cas9 enzyme, and how off-target effects are minimized. Any insights into recent enhancements or challenges would be appreciated.

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